• To state that amino acids react with each other to form proteins and form peptide bonds during this reaction
• To test the presence or absence of proteins qualitatively with the help of Biuret reagent
• To prepare standard solutions using a protein stock solution
• To acquire the absorbance values of the prepared standard solutions using Biuret reagent and to plot a calibration curve
• To calculate the concentration of protein solution with an unknown concentration by using the equation of the calibration curve
LS1-6 From Molecules to Organisms: Structures and Processes
2.1 Testing for biological molecules
2.1 Molecules to metabolism
Categories: Medical Biochemistry Laboratory
Proteins are biomolecules that are responsible for many biological pathways. They are made up of amino acids, and can be detected by Biuret reagent. Biuret reagent reacts with the peptide bonds and detects the presence of proteins. In this experiment, you will conduct a quantitative analysis using bovine serum albumin, Biuret reagent, and a spectrophotometer.
Determine the double bonds in fatty acids qualitatively and make the qualitative determination of cholesterol by a chemical method.
Classify carbohydrates and define terms reducing sugar and glycosidic bond. Fehling reaction will be depending on the properties of the saccharides.