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Spectrophotometry Experiment



• .To perform dilutions

• To use a spectrophotometer

• To distinguish the wavelength of minimum %T for a solution

• To relate light transmission with the concentration of a solution

• To formulate a way to find the concentration of blue dye in a sports drink

• To identify origins of experimental error.


Curriculum alignments


HS-PS4-4: Evaluate the validity and reliability of claims in published materials of the effects that different frequencies of electromagnetic radiation have when absorbed by matter.

• AP

3.11 Spectroscopy and the Electromagnetic Spectrum





Spectroscopy examines interaction between light and matter. As light passes through a solution, some of its energy is trapped by the matter in the solution. This leads to a decrease in light intensity. In this experiment, we will plot a standard graph by measuring the absorption of solutions of known concentrations. Then, we will use this graph to find the concentration of an unknown solution.