•To learn and apply Lambert-Beer Law.
•To learn the types of spectrophotometrical measurements.
•To find the average change in absorbance in a minute by conducting spectrophotometrical analysis.
•To gain knowledge and insight on the enzyme kinetics of arylesterase.
Categories: Medical Biochemistry Laboratory
Spectrophotometrical analysis is used in chemistry, biology and physics experiments to determine the concentration of a solution. It can be utilized to measure the activity of an enzyme or the rate of a reaction. It is based on Lambert-Beer law. Let's find the kinetic enzyme activity of arylesterase by conducting a spectrophotometric analysis.
Determine the double bonds in fatty acids qualitatively and make the qualitative determination of cholesterol by a chemical method.
Classify carbohydrates and define terms reducing sugar and glycosidic bond. Fehling reaction will be depending on the properties of the saccharides.