• To list the steps of protein isolation procedure
• To describe the concepts of lysis and homogenization
• To apply the steps of protein isolation procedure successfully to isolate cytoplasmic protein from liver tissue
HS-LS4-1 Communicate scientific information that common ancestry and biological evolution are supported by multiple lines of empirical evidence.
6.8 Biotechnology
3.5 Genetic modification and biotechnology
19.1 Principles of genetic technology
Categories: Medical Biochemistry Laboratory
Proteins can be found in various places of the cell. There are membrane proteins, cytoplasmic proteins, proteins within organelles and endoplasmic reticulum, as well as proteins that secreted to the extracellular matrix. In this experiment, you will isolate cytoplasmic proteins of liver tissue by homogenization with ultrasonicator and lysis buffer.
Determine the double bonds in fatty acids qualitatively and make the qualitative determination of cholesterol by a chemical method.
Classify carbohydrates and define terms reducing sugar and glycosidic bond. Fehling reaction will be depending on the properties of the saccharides.